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| Santos,Roberta Tancredi Francesco dos; Silva,Marcelo Florencio Passos; Porto,Rafael Marques; Lebrun,Ivo; Gonçalves,Luís Roberto de Camargo; Batista,Isabel de Fátima Correia; Sandoval,Maria Regina Lopes; Abdalla,Fernando Maurício Francis. |
| Abstract Background: Here, we described the presence of a neurotoxin with phospholipase A2 activity isolated from Micrurus lemniscatus venom (Mlx-8) with affinity for muscarinic acetylcholine receptors (mAChRs). Methods: The purification, molecular mass determination, partial amino acid sequencing, phospholipase A2 activity determination, inhibition of the binding of the selective muscarinic ligand [3H]QNB and inhibition of the total [3H]inositol phosphate accumulation in rat hippocampus of the Mlx-8 were determined. Results: Thirty-one fractions were collected from HPLC chromatography, and the Mlx-8 toxin was used in this work. The molecular mass of Mlx-8 is 13.628 Da. Edman degradation yielded the following sequence:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscarinic receptors; Hippocampus; Micrurus lemniscatus; Inositol phosphate; Phopholipase A2. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100301 |
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| Rodrigues-Alves,Patrícia de Sá e Benevides; Flório,Jorge Camilo; Lebrun,Ivo; Bernardi,Maria Martha; Spinosa,Helenice de Souza. |
| The present study investigated the effects of t moxidectin (MXD) in some parameters of rat motor function and neurochemical. The general activity in the open field and the motor coordination in the wooden beam were employed to evaluate the MXD effects. The results showed that, in the open field, even at high doses (2.0 and 20.0 mg/kg), the MXD did not alter the locomotion and the rearing frequencies. However, MXD was able to impair the motor coordination of the animals at wooden beam. Neurochemical studies of striatal GABA and dopamine neurotransmitters showed a reduced levels of dopamine and its metabolite, homovanillic acid, without interference on striatal GABA levels. Since GABAergic receptor stimulation had an inhibitory effect on dopaminergic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Moxidectin; GABA; Motor coordination; Rat; Open field; Wooden beam. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000400012 |
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| Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado. |
| Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016 |
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